Overly honest methods – how experiments really get done

January 7, 2013 § 28 Comments

  1. Plasmids were a gracious gift from the Miser lab after many emails, phone calls, & drunken reminders at conferences. #overlyhonestmethods
  2. Well we had to do *something* with that free sample we scored at a vendor booth #overlyhonestmethods
  3. We decided to use Technique Y because it’s new and sexy, plus hot and cool. And because we could. #overlyhonestmethods
  4. We are reporting four significant figures because, um, wait, how do significant figures work again? #overlyhonestmethods
  5. Heat shock of E.coli was performed at 42-43 degree C for exactly 45-120 seconds. #overlyhonestmethods
  6. “Experiment was repeated until we had three statistically significant similar results and could discard the outliers” #overlyhonestmethods
  7. We used this method because the buffers don’t need to be pH-ed. #overlyhonestmethods
  8. This dye was selected because the bottle was within reach #overlyhonestmethods
  9. incubation lasted three days because this is how long the undergrad forgot the experiment in the fridge #overlyhonestmethods
  10. We study this signaling pathway because it has the best antibodies #overlyhonestmethods
  11. Slices were left in a formaldehyde bath for over 48 hours, because I put them in on Friday and refuse to work weekends. #overlyhonestmethods

§ 28 Responses to Overly honest methods – how experiments really get done

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